Thermo Fisher Scientific KLRG1 Monoclonal Antibody (13F12F2), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific KLRG1 Monoclonal Antibody (13F12F2), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Alternative Formats

• Alexa Fluor 488
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 786
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation available

Product Specific Information

This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitory receptor of the C-type lectin superfamily containing an ITIM domain.
It is expressed on subsets of γδ T cells, NK (CD56dim), CD8+, and CD4+ T cells, primarily on effector/memory phenotype cells that are short-lived but capable of immediate effector function.
Cadherin-E, -N, and -R are ligands for KLRG1, and their interaction inhibits cytolytic activity and proliferation.

Clone 13F12F2 and 13A2 appear to recognize similar epitopes based on cross-blocking studies.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells. Use at 5 µL (1.0 µg) per test (defined for 100 µL final volume and 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm, suitable for cytometers with a violet (405 nm) laser. Ensure instrument compatibility.

For panels containing multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.

Fixation Compatibility: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family containing ITIM motifs that interact with SH2 domains of phosphatases such as SHP-1.
It acts as an inhibitory receptor expressed on NK cells and certain T cells, involved in tumor and virus-infected cell lysis and immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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