Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), eFluor 570, eBioscience
마우스 F4/80 항원을 인식하는 BM8 클론 단클론 항체로, eFluor 570 형광으로 표지되어 있습니다. 성숙 대식세포 표지에 적합하며 IHC, ICC, Flow Cytometry 등 다양한 응용 가능. 555/570 nm 파장에서 발광하며, 4°C 암소 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 153 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | 4 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL | 5 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 37 publications |
| Flow Cytometry (Flow) | - | 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BM8 |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573610 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 661
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 506
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor belonging to the EGF-TM7 family. It contains seven EGF-like domains, seven transmembrane sequences, and a cytoplasmic domain homologous to TM7 proteins. The F4/80 antigen is a key marker for mature macrophages but is also found on peritoneal eosinophils, Langerhans cells, and certain dendritic cell subsets. Expression begins early in myeloid development and can be upregulated by M-CSF stimulation. Some macrophage populations, particularly in lymphoid tissues, may lack F4/80 expression.
Applications Reported:
This antibody is suitable for immunohistochemistry (frozen and paraffin-embedded tissues) and microscopy.
Applications Tested:
Validated for IHC with formalin-fixed paraffin-embedded tissue using pronase antigen retrieval at ≤5 µg/mL. Optimal titration is recommended.
Filter Recommendation:
Use a filter capturing the 570 nm emission wavelength (e.g., Excitation 545/25, 565LP, Emission 605/70). Compatible with standard Alexa Fluor® 555 or TRITC filters.
Excitation/Emission: 555 nm / 570 nm
Target Information
F4/80 antigen is a 160 kDa cell surface glycoprotein, part of the EGF-TM7 family, sharing 68% amino acid identity with human EMR1. Expression varies with macrophage maturation and activation, found in Kupffer cells, Langerhans cells, microglia, gut macrophages, and others. Absent in macrophages of T-cell areas in spleen and lymph nodes. The biological function remains under study, but evidence suggests a role in generating efferent CD8⁺ regulatory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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