Thermo Fisher Scientific CaV1.2 Monoclonal Antibody (S57), PE
CaV1.2 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, PE 형광 표지되어 세포 분석 및 면역염색에 적합. 다양한 종(햄스터, 인간, 생쥐, 랫드)에 반응하며, 단백질 G로 정제된 고순도 항체. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S57 |
| Immunogen | Fusion protein amino acids 1507–1733 (intracellular carboxyl terminus) of rabbit Cav1.2 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.46 mM phosphate / 2.48 mM MES, pH 7.4, with 2 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931844 |
Additional Formats
- Unconjugated (MA5-27717)
- APC (MA5-45387)
- FITC (MA5-45388)
- PerCP (MA5-45389)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45390 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 240 kDa (varies with cell background due to glycosylation).
Target Information
CACNA1C encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Multiple isoforms exist due to alternative splicing or different genes. The encoded protein binds to and is inhibited by dihydropyridine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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