
Thermo Fisher Scientific CaV1.2 Monoclonal Antibody (S57), APC
CaV1.2 단백질을 인식하는 Mouse monoclonal 항체로, APC 형광 표지되어 있습니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합하며, 인간, 생쥐, 햄스터, 랫드 시료에 반응합니다. 단백질 G 정제, 보존제 무첨가, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:200 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S57 |
| Immunogen | Fusion protein amino acids 1507–1733 (intracellular carboxyl terminus) of rabbit Cav1.2 |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931841 |
Available Formats
- Unconjugated (MA5-27717)
- FITC (MA5-45388)
- PE (MA5-45390)
- PerCP (MA5-45389)
- Request custom conjugation
Product Specific Information
1 µg/mL of MA5-45387 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 240 kDa (varies with cell background due to glycosylation).
Target Information
Gene: CACNA1C
CACNA1C encodes an alpha-1 subunit of a voltage-dependent calcium channel. These channels mediate calcium ion influx upon membrane depolarization. The alpha-1 subunit forms the ion-conducting pore and complexes with alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Multiple isoforms exist due to alternative splicing. The protein is inhibited by dihydropyridine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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