Thermo Fisher Scientific CaV1.2 Monoclonal Antibody (S57), FITC
CaV1.2 단백질을 인식하는 S57 클론의 FITC 결합 단일클론 항체입니다. WB, IHC, ICC, IP 등 다양한 응용에 적합하며, 인간·마우스·랫 등에서 반응합니다. 498/517 nm 형광 특성, Protein G 정제, 4°C 암소 저장 조건을 가집니다.
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Thermo Fisher Scientific CaV1.2 Monoclonal Antibody (S57), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:200 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S57 |
| Immunogen | Fusion protein amino acids 1507–1733 (intracellular carboxyl terminus) of rabbit Cav1.2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931842 |
Additional Formats
- Unconjugated (MA5-27717)
- APC (MA5-45387)
- PE (MA5-45390)
- PerCP (MA5-45389)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45388 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 240 kDa (varies with cell background due to glycosylation).
Target Information
CACNA1C encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization.
The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell.
The calcium channel is composed of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio.
Multiple isoforms exist due to alternative splicing or different gene encoding.
The CACNA1C protein binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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