Thermo Fisher Scientific AMPK alpha 1 Polyclonal Antibody
AMPK alpha 1 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, IP 등에 사용 가능. Human 및 Mouse에 반응하며 항원 친화 크로마토그래피로 정제됨. 액상 형태로 4°C에서 보관하며 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–8 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval is not recommended
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan
Target Information
Protein kinases transfer a phosphate group from ATP to an acceptor amino acid in substrate proteins, mediating signal transduction in eukaryotic cells. They regulate metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation. The protein kinase family, with over 500 gene products, is one of the largest in eukaryotes and is classified into 8 major groups based on catalytic domain sequence comparison.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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