Thermo Fisher Scientific IL-3 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein
MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC.

Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-3.
Anti-Murine IL-3-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-3 matrix.

Sandwich ELISA
To detect Murine IL-3 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required.
This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-3 (500-P53Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-3.

Western Blot
To detect Murine IL-3 by Western Blot analysis, use at 0.1–0.2 µg/mL.
With compatible secondary reagents, the detection limit for Recombinant Murine IL-3 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils.
IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Most of these functions are enhanced or dependent on co-stimulation with other cytokines.
Human and mouse IL-3 share only 29% sequence identity at the amino acid level, and IL-3 bioactivity is highly species specific — human IL-3 has no activity on mouse cells.
IL-3 supports proliferation of a broad range of hematopoietic cell types and is involved in cell growth, differentiation, and apoptosis.
It also possesses neurotrophic activity and may be associated with neurologic disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.