Thermo Fisher Scientific IL-4 Polyclonal Antibody, PeproTech
마우스 IL-4 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ELISA 등 다양한 면역학적 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 무보존 PBS 용액 상태로 동결건조되어 제공됩니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P54-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-4 Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| Immunohistochemistry (IHC) | – | 1 publication |
| Flow Cytometry (Flow) | – | 3 publications |
| ELISA | 0.5–2.0 µg/mL | 1 publication |
| Neutralization (Neu) | – | 1 publication |
| In vitro Assay (IV) | – | 14 publications |
| Immunostaining (IS) | – | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-4 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930617 |
Product Specific Information
AA Sequence of recombinant protein:
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDYS
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-4. Anti-Murine IL-4-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-4 matrix.
Sandwich ELISA:
To detect Murine IL-4 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Murine IL-4 (500-P54Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-4.
Western Blot:
To detect Murine IL-4 by Western Blot, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine IL-4 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, serving as a ligand for the interleukin 4 receptor. IL-4 exhibits a broad range of biological actions and shares receptor components with IL-13, contributing to overlapping functions. STAT6 plays a crucial role in mediating IL-4 signaling. The IL-4 gene is located on chromosome 5q within a cytokine gene cluster that includes IL-3, IL-5, IL-13, and CSF2. IL-4, IL-13, and IL-5 are co-regulated by long-range elements across approximately 120 kb. A known splice variant, IL-4 delta 2, has also been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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