Thermo Fisher Scientific Cytokeratin 8 Monoclonal Antibody (SP102)
인간 Cytokeratin 8을 인식하는 Rabbit monoclonal antibody로 다양한 면역실험(WB, IHC, ICC, Flow)에 사용 가능. 단백질 A/G로 정제된 액상 형태이며, PBS 버퍼에 1% BSA 및 sodium azide 포함. 세포 구조 및 분화 연구용으로 적합.
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Thermo Fisher Scientific Cytokeratin 8 Monoclonal Antibody (SP102)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:20 | - |
| Immunocytochemistry (ICC/IF) | 1:25–1:100 | - |
| Flow Cytometry (Flow) | 1:200 | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP102 |
| Immunogen | Synthetic peptide corresponding to C-terminus of human keratin 8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.01 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537939 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is breast carcinoma tissue.
Target Information
Cytokeratin 8 is a member of the type II keratin family located on chromosome 12. Type I and II keratins form intermediate filaments in epithelial cells.
Cytokeratin 8 typically dimerizes with keratin 18 to form filaments in simple epithelial cells.
It contributes to cellular structural integrity, signal transduction, and differentiation.
Mutations in this gene are associated with cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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