Thermo Fisher Scientific J Chain Monoclonal Antibody (SP105)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

1:100

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Flow Cytometry (Flow)

1:20

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Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP105

Immunogen

Synthetic peptide near the internal region of human J-chain.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Purification

Protein A/G

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537938

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.

Target Information

J chain is a small glycopeptide and is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells that secrete polymeric immunoglobulins. J chain is linked to IgA and IgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsil and lymph node. B cells secrete J chain at an early stage of differentiation with the expression persisting in those cells destined to produce IgA or IgM.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.