Thermo Fisher Scientific Nestin Monoclonal Antibody (SP103)
인간 Nestin 단백질을 인식하는 Rabbit monoclonal 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 단백질 A/G로 정제된 액상 형태이며, PBS 버퍼에 1% BSA 포함. 줄기세포 및 종양 혈관 신생 연구에 적합.
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Thermo Fisher Scientific Nestin Monoclonal Antibody (SP103)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP103 |
| Immunogen | Synthetic peptide corresponding to C-terminus of human nestin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537940 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes. After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is kidney tissue.
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system and early embryonic neuroepithelial stem cells. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin is also a superior angiogenic marker for assessing neovascularity in tumors. Highly specific antibodies to human Nestin can help characterize progenitor cell differentiation, enhance therapeutic potential of neural stem/progenitor cells, and identify neuroepithelial tumor cells.
Nestin expression has also been observed in pancreatic hematopoietic stem cells and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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