Thermo Fisher Scientific Nestin Monoclonal Antibody (SP103)
인간 Nestin 단백질을 인식하는 Rabbit monoclonal 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 단백질 A/G로 정제된 액상 형태이며, PBS 버퍼에 1% BSA 포함. 줄기세포 및 종양 혈관 신생 연구에 적합.
- 카탈로그번호
- MA516421
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Nestin Monoclonal Antibody (SP103)
Thermo Fisher Scientific Nestin Monoclonal Antibody (SP103)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP103 |
| Immunogen | Synthetic peptide corresponding to C-terminus of human nestin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537940 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes. After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is kidney tissue.
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system and early embryonic neuroepithelial stem cells. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin is also a superior angiogenic marker for assessing neovascularity in tumors. Highly specific antibodies to human Nestin can help characterize progenitor cell differentiation, enhance therapeutic potential of neural stem/progenitor cells, and identify neuroepithelial tumor cells.
Nestin expression has also been observed in pancreatic hematopoietic stem cells and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.