Takara Embgenix PGT-A Kit (RUO)

Takara Embgenix PGT-A Kit (RUO)

The Embgenix PGT-A Kit (RUO) and accompanying Embgenix Analysis Software provide a complete solution for next-generation sequencing (NGS)-based detection of chromosomal copy number variations (CNVs) from embryo biopsy samples.
This kit enables accurate identification of whole chromosome, segmental, and mosaic aneuploidies, and yields Illumina-compatible sequencing libraries in a scalable format to support medium- to high-throughput applications.

Key Features

• Complete solution for NGS-based CNV detection, including WGA, library preparation, and bioinformatic analysis
• Reliable detection of CNVs from trophectoderm (TE) biopsy samples, including whole-chromosome and segmental gains/losses ≥10 Mb and mosaicisms ≥30%
• Powered by industry-leading PicoPLEX WGA technology, providing unparalleled reproducibility and coverage uniformity
• Scalable library prep workflow requiring about two hours of hands-on time and allowing multiplexing of up to 96 samples
• Broad compatibility with Illumina sequencing platforms; validated for MiSeq® and NextSeq® 500/550
• Intuitive, cloud-based analysis software for streamlined processing and reporting of sequencing data

Technology Overview

The kit employs PicoPLEX technology for highly reproducible whole genome amplification (WGA) and a streamlined library preparation methodology that minimizes hands-on time and sample processing errors.
The entire workflow can be completed in a single tube in approximately two hours, producing Illumina-compatible NGS libraries that support multiplexing of up to 96 samples per sequencing run.

Data Analysis

After sequencing, cloud-based Embgenix Analysis Software (RUO) is used for data analysis and reporting.
The software algorithm computes calculated copy numbers (CCNs) using bin counts against an internal reference for accurate CNV determination.
It supports both automated and manual calling of whole chromosome, segmental, and mosaic CNVs, with visualization options such as charts and idiogram plots.
Additional features include bulk download of results and customizable PDF report generation.

Applications

• Detection of CNVs in gDNA and embryo samples
• Reproductive health research

Additional Product Information

Refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
The Product Components List is available under the Documents tab.

Specifications

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