Thermo Fisher Scientific LOK Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:15,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.

Target Information

Ubiquitin exists either covalently attached to another protein or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues (polyubiquitin chains), or a linear polymer linked via the initiator Met (linear polyubiquitin chains).
Polyubiquitin chains have distinct functions depending on the Lys residue linkage:

• Lys-6: DNA repair
• Lys-11: ERAD and cell-cycle regulation
• Lys-29: Lysosomal degradation
• Lys-33: Kinase modification
• Lys-48: Proteasomal degradation
• Lys-63: Endocytosis, DNA-damage responses, NF-kappa-B activation

Linear polymer chains formed via attachment by the initiator Met lead to cell signaling.
Ubiquitin is usually conjugated to Lys residues of target proteins, though rare cases involve Cys or Ser residues. Free polyubiquitin also plays roles in activation of protein kinases and signaling.

60S ribosomal protein L40: Component of the 60S subunit of the ribosome. Essential for translation of specific transcripts and cap-dependent translation of vesicular stomatitis virus mRNAs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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