Thermo Fisher Scientific LOK Polyclonal Antibody
Rabbit 유래 다클론 항체로 Western blot 및 Immunoprecipitation에 적합. 인간 LOK 단백질의 C-말단 영역(900–968 aa)을 면역원으로 제작. 1 mg/mL 농도의 액상 형태로 제공되며 4°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:15,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 900 and the C-terminus (residue 968) of human Lymphocyte Oriented Kinase (Serine/Threonine Kinase 10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
Target Information
Ubiquitin exists either covalently attached to another protein or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond as monoubiquitin or polyubiquitin chains. Polyubiquitin chains have different functions depending on the Lys residue linkage:
- Lys-6: DNA repair
- Lys-11: ERAD and cell-cycle regulation
- Lys-29: Lysosomal degradation
- Lys-33: Kinase modification
- Lys-48: Protein degradation via proteasome
- Lys-63: Endocytosis, DNA-damage response, NF-κB activation
Linear polymer chains via Met linkage are involved in cell signaling.
Free polyubiquitin also plays roles in kinase activation and signaling.
60S ribosomal protein L40 is a component of the 60S ribosomal subunit, essential for translation of specific cellular transcripts, particularly for cap-dependent translation of vesicular stomatitis virus mRNAs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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