Thermo Fisher Scientific Nanog Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2.

Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research has shown that high levels of human NANOG expression occur in the undifferentiated N-Tera embryonal carcinoma cell line. NANOG acts as a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. Its role in embryonic development suggests utility in generating stem cells for potential cell replacement therapies in degenerative diseases.

Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (Oct-4), Sox2, Klf4, Lin28, and c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, Klf4, and c-Myc, eliminating the need for viral introduction, addressing safety concerns for regenerative medicine. When overexpressed, NANOG promotes cells to enter S phase and proliferation. Diseases associated with NANOG dysfunction include teratocarcinoma and germ cell/embryonal cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.