Thermo Fisher Scientific Phospho-PKC gamma (Thr514) Recombinant Rabbit Monoclonal Antibody (PKCgT514-PF4), PE
Phospho-PKC gamma (Thr514)을 인식하는 재조합 토끼 단클론 항체로, PE 형광표지되어 있습니다. 사람, 마우스, 랫트 시료에 반응하며 Flow Cytometry에 최적화되어 있습니다. 단백질 A/G로 정제되었으며, 4°C에서 보관합니다.
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Applications
- Flow Cytometry (Flow): 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PKCgT514-PF4 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr514 of human phospho PKC gamma. |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.20% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897127 |
Additional Formats
Target Information
The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. PKC also serves as a major receptor for phorbol esters, a class of tumor promoters.
PKC gamma is expressed solely in the brain and spinal cord, localized to neurons. It plays roles in neuronal functions such as long-term potentiation (LTP) and long-term depression (LTD). Knockout studies in mice suggest involvement in neuropathic pain development. Defects in this protein are associated with the neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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