Thermo Fisher Scientific Phospho-PKC gamma (Thr514) Recombinant Rabbit Monoclonal Antibody (PKCgT514-PF4), APC
APC 형으로 결합된 재조합 rabbit monoclonal anti-Phospho-PKC gamma (Thr514) 항체로, 인간·마우스·랫트 시료에 반응합니다. Flow Cytometry에 적합하며, HEK293 발현 시스템에서 생산되었습니다. 뇌 및 척수에서 발현되는 PKCγ 신호전달 연구에 활용됩니다.
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Applications
- Flow Cytometry (Flow): 5 µL/1x10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PKCgT514-PF4 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr514 of human phospho PKC gamma |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage buffer | PBS with 0.20% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4° C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897129 |
Additional Formats
- Unconjugated (MA5-37192)
- FITC (MA5-37194)
- PE (MA5-37193)
- Custom conjugation available on request
Target Information
The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in structure, tissue distribution, localization, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent.
Protein kinase C can be activated by calcium and diacylglycerol and phosphorylates diverse protein targets involved in cellular signaling. PKC family members also act as receptors for phorbol esters, tumor promoters. PKC gamma is expressed solely in the brain and spinal cord, localized to neurons, and involved in neuronal functions such as long-term potentiation (LTP) and long-term depression (LTD). Knockout studies suggest its role in neuropathic pain development. Defects in PKC gamma are associated with spinocerebellar ataxia-14 (SCA14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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