Thermo Fisher Scientific CD137 (4-1BB) Monoclonal Antibody (4B4 (4B4-1)), Alexa Fluor 700, eBioscience
인간 CD137(4-1BB) 인식 단클론 항체로서 T세포 활성화 및 면역 조절 연구에 적합. Alexa Fluor 700 형광 표지로 유세포분석에 최적화. 고순도 친화 크로마토그래피 정제, 4°C 암소 보관. 연구용으로만 사용.
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Thermo Fisher Scientific CD137 (4-1BB) Monoclonal Antibody (4B4 (4B4-1)), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 3 publications |
| ELISA | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B4 (4B4-1) |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848466 |
Available Formats
- APC
- APC-eFluor 780
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
This 4B4 (4B4-1) monoclonal antibody reacts with human CD137 (also known as 4-1BB or TNFRSF9), an inducible member of the TNFR family of costimulatory molecules expressed on T cells, NK cells, dendritic cells, granulocytes, and mast cells.
CD137 signaling recruits TRAF1 and TRAF2, playing roles in T cell activation, survival of activated and CD8 memory T cells, and suppression of myelopoiesis and dendritic cell development.
Stimulation of CD137 promotes expansion of CD4+CD25+ regulatory T cells ex vivo.
Its ligand, 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometry using stimulated normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume).
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited by red laser (633–647 nm).
Recommended optics: 685 LP mirror and 710/20 band pass filter.
Ensure instrument compatibility for detection.
Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser
Target Information
CD137 (TNFRSF9, 4-1BB) is an inducible costimulatory molecule mainly expressed on activated T cells.
Its ligand, 4-1BBL, is found on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.
CD137 signaling supports survival of activated and memory T cells and promotes T cell clonal expansion, while suppressing myelopoiesis and dendritic cell development.
Triggered CD137 induces cytokine release regulating peripheral monocyte survival.
Soluble CD137 may act as a negative regulator for some immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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