Thermo Fisher Scientific CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), Alexa Fluor 700, eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.25 µg)/test
• View 15 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 711
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and possibly by hematopoietic progenitor cells. It functions as a high-affinity scavenger receptor for the hemoglobin-haptoglobin complex, facilitating clearance of hemoglobin from the bloodstream.

Upon ligand binding, CD163 triggers signaling leading to IL-6 and IL-10 production. Expression of CD163 is induced by anti-inflammatory cytokines (IL-6, IL-10) and downregulated by proinflammatory stimuli (LPS, TNF-α), which can cause shedding of soluble CD163.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test in 100 µL final volume. Suitable for 10⁵–10⁸ cells/test.

Fluorochrome Information:

• Excitation: 633–647 nm
• Emission: 723 nm
• Laser: Red Laser
• Recommended filter: 685 LP mirror and 710/20 bandpass filter

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein of the scavenger receptor cysteine-rich superfamily. It serves as a receptor for the hemoglobin-haptoglobin complex, protecting tissues from oxidative damage and aiding in iron recycling.

CD163 is expressed on monocytes and macrophages, particularly during late inflammation. It is found on CD14⁺ and CD64⁺ monocytes, with higher expression on CD16⁺ subsets. Expression is upregulated by IL-10, IL-6, and dexamethasone, and downregulated by LPS or PMA through shedding into plasma.

Binding of CD163 to hemoglobin/haptoglobin complexes is calcium- and pH-dependent, showing higher affinity for multimeric haptoglobin (HP1F phenotype). It also induces intracellular signaling involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL-6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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