Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• NovaFluor Red 700
• NovaFluor Red 710
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 645
• Request custom conjugation

Product Specific Information

The A7R34 monoclonal antibody reacts with mouse CD127, the high-affinity alpha subunit of the mouse IL-7 receptor.
IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes.
In the periphery, mature T cells express CD127 at low levels. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation.
Binding of A7R34 blocks the binding of SB/199, another antibody recognizing mouse CD127.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse thymocyte and splenocyte suspensions, ≤1 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Antibody should be titrated for optimal performance.

Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm and is excited with a red laser (633 nm). Recommended filters: 685 LP mirror and 710/20 filter.

• Excitation: 633–647 nm
• Emission: 723 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD127 (Interleukin-7 receptor alpha, IL-7Rα) is a glycoprotein involved in lymphopoiesis regulation.
The active IL7R receptor is an alpha/gamma chain heterodimer. The gamma(c) chain activates signal transduction, while the alpha chain determines specific signaling events.
CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells.
Defects in CD127 function are associated with severe combined immunodeficiency (SCID) and T⁻B⁻NK⁺ SCID.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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