Thermo Fisher Scientific CD127 Monoclonal Antibody (A7R34), Alexa Fluor 700, eBioscience
마우스 CD127(IL-7Rα)을 인식하는 A7R34 단클론 항체로, Alexa Fluor 700으로 표지되어 있습니다. Flow cytometry에 적합하며, IL-7 수용체 결합 억제 연구에 활용됩니다. 0.2 mg/mL 농도의 액상 제품이며, 4°C 암조건에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 25 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A7R34 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657613 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- NovaFluor Red 700
- NovaFluor Red 710
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Request custom conjugation
Product Specific Information
The A7R34 monoclonal antibody reacts with mouse CD127, the high-affinity alpha subunit of the mouse IL-7 receptor.
IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes.
In the periphery, mature T cells express CD127 at low levels. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation.
Binding of A7R34 blocks the binding of SB/199, another antibody recognizing mouse CD127.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse thymocyte and splenocyte suspensions, ≤1 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Antibody should be titrated for optimal performance.
Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm and is excited with a red laser (633 nm). Recommended filters: 685 LP mirror and 710/20 filter.
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD127 (Interleukin-7 receptor alpha, IL-7Rα) is a glycoprotein involved in lymphopoiesis regulation.
The active IL7R receptor is an alpha/gamma chain heterodimer. The gamma(c) chain activates signal transduction, while the alpha chain determines specific signaling events.
CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells.
Defects in CD127 function are associated with severe combined immunodeficiency (SCID) and T⁻B⁻NK⁺ SCID.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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