Thermo Fisher Scientific CXCL16 Polyclonal Antibody, Biotin, PeproTech

Applications

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
NQGSVAGSCS CDRTISSGTQ IPQGTLDHIR KYLKAFHRCP FFIRFQLQSK SVCGGSQDQW VRELVDCFER KECGTGHGKS FHHQKHLP

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine CXCL16. Anti-Murine CXCL16-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mCXCL16 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine CXCL16 (500-P201G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant mCXCL16.

Western Blot:
To detect Murine CXCL16 by Western Blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, detection limit for Recombinant Murine CXCL16 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P201GBT-1MG is provided as 2 × 500 µg.

Target Information

CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor. It may play a role in attracting lymphocyte subsets during inflammation and facilitate certain immune responses.
The chemokine domain of CXCL16 contains six cysteine residues, including four highly conserved ones characteristic of CXC chemokines.
The CXCL16 gene codes for a 273 amino acid polypeptide, including a 29 amino acid cytoplasmic domain and a transmembrane sequence (~20 amino acids).
The extracellular portion contains a chemokine domain and an extended C-terminal mucin-like stalk sequence, with 89 amino acid residues (86 for murine homolog).

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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