Thermo Fisher Scientific CXCL16 Polyclonal Antibody, PeproTech
마우스 CXCL16 단백질을 인식하는 산염기 친화 정제된 염소 다클론 항체입니다. Western blot, ELISA, 면역염색에 적합하며, 항원은 E.coli 유래 재조합 단백질입니다. PBS 용액 내 동결건조 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | - |
| Immunostaining (IS) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine CXCL16 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929790 |
Product Specific Information
Amino Acid Sequence (recombinant protein):
NQGSVAGSCS CDRTISSGTQ IPQGTLDHIR KYLKAFHRCP FFIRFQLQSK SVCGGSQDQW VRELVDCFER KECGTGHGKS FHHQKHLP
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine CXCL16. The anti-Murine CXCL16-specific antibody was purified by affinity chromatography using an immobilized Murine CXCL16 matrix.
Sandwich ELISA:
To detect Murine CXCL16 by sandwich ELISA, use 0.5–2.0 µg/mL of this antibody (100 µL/well). In conjunction with PeproTech Biotinylated Anti-Murine CXCL16 (500-P201GBt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Murine CXCL16.
Western Blot:
For detection of Murine CXCL16 by Western blot, use 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine CXCL16 is 1.5–3.0 ng/lane under both reducing and non-reducing conditions.
Target Information
CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor. It may play a role in attracting lymphocyte subsets during inflammation and facilitating immune responses.
The CXCL16 gene encodes a 273 amino acid polypeptide, including a 29 amino acid cytoplasmic domain and a transmembrane sequence (~20 amino acids). The extracellular domain contains a chemokine domain and a mucin-like stalk sequence, comprising 89 amino acid residues (86 for the murine homolog).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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