Thermo Fisher Scientific IL-10 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 3 publications 3 publications

Neutralization (Neu)

-

View 3 publications 3 publications

In vitro Assay (IV)

-

View 4 publications 4 publications

Immunostaining (IS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IL-10 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL-10,uniProtId:P22301-1,ncbiNodeId:9606,antigenRange:19-178,antigenLength:178,antigenImageFileName:500-P20-100UG_IL-10_P22301-1_Rabbit.svg,antigenImageFileNamePDP:500-P20-100UG_IL-10_P22301-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929789

Product Specific Information

AA Sequence of recombinant protein: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified by affinity chromatography employing an immobilized Human IL-10 matrix.

Sandwich ELISA: To detect Human IL-10 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-10 (500-P20Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-10.

Western Blot: To detect Human IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.