Thermo Fisher Scientific IL-10 Polyclonal Antibody, PeproTech
Human IL-10을 특이적으로 인식하는 Rabbit Polyclonal Antibody로, Western Blot, ELISA, IHC 등에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 순도 제공. Lyophilized 형태로 -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (IHC) | – | 1 publication |
| ELISA | 0.5–2.0 µg/mL | 3 publications |
| Neutralization (Neu) | – | 3 publications |
| In vitro Assay (IV) | – | 4 publications |
| Immunostaining (IS) | – | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-10 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929789 |
Product Specific Information
AA Sequence of recombinant protein:
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified by affinity chromatography employing an immobilized Human IL-10 matrix.
Sandwich ELISA:
To detect Human IL-10 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-10 (500-P20Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-10.
Western Blot:
To detect Human IL-10 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-10 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-10 encodes a cytokine primarily produced by monocytes and lymphocytes. It plays a key role in immunoregulation and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival, proliferation, and antibody production, blocks NF-kappa B activity, and regulates the JAK-STAT signaling pathway. Knockout studies indicate IL-10 is essential for intestinal immune regulation. Mutations are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis. Associated diseases include Graft-Versus-Host Disease and HIV-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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