Thermo Fisher Scientific YY1 Recombinant Rabbit Monoclonal Antibody (6J6L1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

-

Immunocytochemistry (ICC/IF)

1:50-1:400

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

6J6L1

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human YY1 (P25490). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:YY1,uniProtId:P25490-1,ncbiNodeId:9606,antigenRange:200-300,antigenLength:414,antigenImageFileName:MA5-42708_YY1_P25490-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42708_YY1_P25490-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911849

Product Specific Information

Positive test controls include: Mouse liver, Mouse kidney, Mouse spleen, Rat spleen, U-251MG, HeLa, Jurkat, Raji. The target is usually found in the following locations: Nucleus matrix.

Immunogen sequence: PGNKKWEQKQ VQIKTLEGEF SVTMWSSDEK KDIDHETVVE EQIIGENSPP DYSEYMTGKK LPPGGIPGID LSDPKQLAEF ARMKPRKIKE DDAPRTIACP H

Target Information

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.