Thermo Fisher Scientific ERK1 Recombinant Rabbit Monoclonal Antibody (0B0C1)
ERK1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성, 고순도 친화 크로마토그래피 정제, 안정적인 액상 형태로 연구용에 최적화된 항체입니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:800 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 0B0C1 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human ERK1 (P27361) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.13 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911847 |
Product Specific Information
Positive test controls include: Raji, HepG2, 293T, Jurkat, Mouse kidney, Mouse heart, Rat brain, Rat lung.
Target localization: Cytoplasm, Nucleus.
Immunogen sequence:
MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that activates p90, RSK, MSK, ELK1, and Stat3, and is a key component of the MAPK pathway.
ERK1 regulates meiosis, mitosis, and post-mitotic functions, and is activated by various stimuli such as growth factors, cytokines, and viral infections.
It translocates to the nucleus upon activation to phosphorylate nuclear targets.
Alternative splicing variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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