Thermo Fisher Scientific ALPL Recombinant Rabbit Monoclonal Antibody (SA40-00)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:8,000

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:50

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SA40-00

Immunogen

Synthetic peptide within human Alkaline Phosphatase aa 18-50 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ALPL,uniProtId:P05186-1,ncbiNodeId:9606,antigenRange:18-50,antigenLength:524,antigenImageFileName:MA5-42414_ALPL_P05186-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42414_ALPL_P05186-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2911555

Product Specific Information

Positive Control: Hela cell lysate, HepG2 cell lysate, MG-63 cell lysate, Hela, HepG2, SW480, human liver tissue, human liver carcinoma tissue, human kidney tissue, mouse kidney tissue, mouse small intestine tissue.

Subcellular Location: Cell membrane

Target Information

Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism`s environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.