
Thermo Fisher Scientific ALPL Recombinant Rabbit Monoclonal Antibody (SA40-00)
Rabbit 모노클로날 항체로 ALPL 단백질을 특이적으로 인식. WB, IHC, ICC, Flow, IP 등 다양한 응용에 사용 가능. HEK293 세포 발현, Protein A 정제, 1 mg/mL 농도. 인체, 마우스, 랫트 시료에 반응. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:8,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SA40-00 |
| Immunogen | Synthetic peptide within human Alkaline Phosphatase (aa 18–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911555 |
Product Specific Information
- Positive Control: HeLa cell lysate, HepG2 cell lysate, MG-63 cell lysate, HeLa, HepG2, SW480, human liver tissue, human liver carcinoma tissue, human kidney tissue, mouse kidney tissue, mouse small intestine tissue
- Subcellular Location: Cell membrane
Target Information
Alkaline phosphatase (ALPL) is a homodimeric enzyme (86 kDa) containing two zinc atoms per monomer essential for its catalytic activity. It is optimally active at alkaline pH and is found across prokaryotic and eukaryotic organisms with structural adaptations suited to each environment. ALP plays a key role in liver metabolism and skeletal development. In bacteria, it provides inorganic phosphate, while in yeast, it serves as a model for membrane protein sorting studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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