Thermo Fisher Scientific ARNT Monoclonal Antibody (OTI1D10), TrueMAB
Human ARNT 단백질을 인식하는 Mouse monoclonal antibody로, WB, IHC, ICC에 적합. Lyophilized 형태로 제공되며, 재구성 시 1 mg/mL 농도 유지. Hypoxia 관련 연구 및 HIF-1 beta 단백질 분석에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D10 |
| Immunogen | Full length human recombinant protein of human ARNT produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
HIF-1 beta (ARNT) is part of the aryl hydrocarbon receptor nuclear translocator gene family.
It plays a critical role in oxygen homeostasis and is involved in the cellular response to hypoxia.
HIF-1 is a heterodimer consisting of HIF-1 alpha and HIF-1 beta subunits. Under normoxic conditions, HIF-1 alpha undergoes hydroxylation and degradation, while HIF-1 beta remains stable.
Diseases associated with HIF-1 beta dysfunction include hypoxia-related conditions and renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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