Thermo Fisher Scientific PRKAR2A Monoclonal Antibody (OTI1F8), TrueMAB
PRKAR2A 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. HEK293T 세포에서 생산된 full-length human recombinant protein을 면역원으로 사용. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F8 |
| Immunogen | Full length human recombinant protein of human PRKAR2A produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
cAMP is a signaling molecule important for various cellular functions. It activates the cAMP-dependent protein kinase (AMPK), which phosphorylates target proteins. The inactive holoenzyme of AMPK is a tetramer with two regulatory and two catalytic subunits. Upon cAMP binding, the holoenzyme dissociates into regulatory dimers and free catalytic monomers.
The PRKAR2A gene encodes one of the regulatory subunits, which can be phosphorylated by activated catalytic subunits and interacts with A-kinase anchoring proteins to determine AMPK subcellular localization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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