Thermo Fisher Scientific ARNT Monoclonal Antibody (OTI1D1), TrueMAB
ARNT 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, ICC/IF, Flow cytometry에 적합합니다. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 인간 ARNT 단백질을 면역원으로 제작된 고특이성 항체입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D1 |
| Immunogen | Full length human recombinant protein of human ARNT produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
HIF-1 beta, also known as ARNT (aryl hydrocarbon receptor nuclear translocator), is a nuclear protein involved in mammalian oxygen homeostasis. It forms a heterodimer with HIF-1 alpha, regulating cellular responses to hypoxia.
Under normoxic conditions, HIF-1 alpha is hydroxylated and degraded, while HIF-1 beta remains stable.
Dysfunction of HIF-1 beta has been associated with hypoxia-related diseases, renal cell carcinoma, and other pathological conditions such as myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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