Thermo Fisher Scientific Phospho-AKT (Ser473) Monoclonal Antibody (17F6.B11), Biotin
Phospho-AKT (Ser473) 단백질을 검출하는 Biotin 결합 단일클론 항체. 인간, 마우스, 랫, 비인간 영장류에 반응. Western blot, IHC, ELISA, IP에 사용 가능. Protein A 정제, Lyophilized 형태로 제공되며 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific Phospho-AKT (Ser473) Monoclonal Antibody (17F6.B11), Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL |
| ELISA | 1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17F6.B11 |
| Immunogen | Synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- Atto 594
- DyLight 488
- DyLight 549
- DyLight 649
- Custom conjugation available upon request
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human and mouse AKT protein phosphorylated at S473. BLAST analysis suggests cross-reactivity with AKT pS473 from human, mouse, rat, and chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with AKT2 and AKT3 has not been determined.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. Activated by insulin, PI3K, IGF1, and other growth factors, Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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