Thermo Fisher Scientific MEK2 C-Term Monoclonal Antibody (12A6.G1.G11), HRP
MEK2 단백질의 C-말단을 인식하는 HRP 결합 단일클론 항체로, Western blot 및 ELISA에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 높은 특이성과 재현성을 제공합니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
ELISA
- Tested Dilution: 1:40,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12A6.G1.G11 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the C-terminus of MEK2 conjugated to KLH |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Biotin
- DyLight 680
- DyLight 800
- FITC
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction. MEK2 activates ERK1/2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, acting as a negative feedback loop that suppresses MEK1 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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