Thermo Fisher Scientific Phospho-PKC theta (Ser695) Polyclonal Antibody
Rabbit polyclonal antibody recognizing phospho-PKC theta (Ser695). Validated for WB and IHC (paraffin). High specificity to human PKC-theta phosphorylation site. Supplied as liquid, affinity purified, 1 mg/mL concentration. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 695 (N-F-S(p)-F-M) derived from Human PKC-theta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554305 |
Product Specific Information
Suggested positive control for Western blot: 293 cells
Suggested positive control for IHC: human lung carcinoma
Target Information
Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that regulate differentiation, proliferation, gene expression, brain function, membrane transport, and cytoskeletal organization. PKC acts as a key regulator of cell cycle transitions, including G1 and G2 checkpoints. Twelve PKC isoforms have been identified in humans (α, βI, βII, γ, δ, ε, ζ, η, θ, ι, λ, μ), differing in structure, localization, and tissue distribution. PKC βII is expressed in cardiac muscle, intestinal epithelium, kidney, and pancreas, and is implicated in cell cycle progression and cancer proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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