Thermo Fisher Scientific Phospho-PKC mu (Tyr463) Polyclonal Antibody
Phospho-PKC mu (Tyr463) 폴리클로날 항체로 인간, 마우스, 랫트 반응성. Western blot과 IHC(Paraffin)에 사용 가능. 고순도 친화 크로마토그래피 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-PKC mu (Tyr463) Polyclonal Antibody
Applications
- Western Blot (WB): 1:500–1:1,000
- Immunohistochemistry (Paraffin) (IHC (P)): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine463 (R-Y-Y(p)-K-E) derived from Human PKD1/PKC-mu |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2554303 |
Product Specific Information
A suggested positive control for Western blot is HepG2 cells.
Target Information
The PKC family of serine/threonine kinases, including PRKCN (PKD3), is activated intracellularly by signal transduction pathways.
In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent.
PKC mu activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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