Thermo Fisher Scientific Phospho-MEK3 (Thr222) Polyclonal Antibody
인간 MEK3 Thr222 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(P)에서 사용 가능. 1 mg/mL 농도, PBS/glycerol buffer에 보관. 세포 신호전달 연구용으로 적합.
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Thermo Fisher Scientific Phospho-MEK3 (Thr222) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of threonine 222 (A-K-T(p)-M-D) derived from Human MAP2K3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554307 |
Product Specific Information
- Suggested positive control for Western blot: Jurkat cells
- Suggested positive control for IHC: Human brain tissue
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase involved in cellular signal transduction pathways. It phosphorylates and activates MAPK14/p38-MAPK and is necessary for glucose transporter expression. Activation by insulin and RAS oncogene expression leads to constitutive MAPK14 activation and oncogenic transformation. Inhibition of MEK3 is associated with Yersinia pseudotuberculosis pathogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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