Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.1 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-F1 recognizes CD85j (also known as ILT2, LILRB1, LIR1), a member of the ILT/LIR/MIR receptor family.
CD85j is a transmembrane glycoprotein with four ITIMs that signal through SHP-1 interactions. It is expressed on myeloid cells (monocytes, dendritic cells) and subsets of NK, T, and B cells. Expression correlates with leukemias (ALL, CLL) and viral infections (HIV, EBV, CMV).
Ligands include MHC Class I molecules such as HLA-G, A, F, B27, E, and F.

HP-F1 antibody reduces CD16-dependent cytolytic activity of NK cells.
Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral cells. Recommended ≤5 µL (0.1 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, or DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample prior to combining with cells. For single-color controls, use 5 µL per 100 µL of cell sample.

Technology:
NovaFluor conjugates are based on Phiton™ technology, utilizing nucleic acid dye structures for engineered fluorescent signatures with minimized spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

This gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at chromosome 19q13.4.
It encodes a receptor with extracellular Ig domains, a transmembrane region, and cytoplasmic ITIM motifs.
Expressed on immune cells, it binds MHC class I molecules, transmitting inhibitory signals that regulate immune activation, inflammation, and cytotoxicity.
Multiple transcript variants and isoforms exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.