Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Yellow 570, eBioscience

Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Yellow 570

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The monoclonal antibody HP-F1 recognizes CD85j (ILT2, LILRB1, LIR1), a member of the ILT/LIR/MIR family.
CD85j is a transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs that signal through SHP-1.
Expression is found on myeloid cells (monocytes, dendritic cells) and subsets of NK, T, and B cells.
It is correlated with leukemias (ALL, CLL) and viral infections (HIV, EBV, CMV).
Ligands include MHC Class I molecules such as HLA-G, A, F, B27, E, and F.
HP-F1 has been shown to reduce CD16-dependent cytolytic activity of NK cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on human peripheral blood cells
Use at 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

NovaFluor Compatibility:

• Not compatible with DNA intercalating viability dyes (propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for NovaFluor dyes.

CellBlox Plus Blocking Buffer:
Updated formulation (Cat. No. C001T06F01) for optimal staining.
Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Dyes:
Based on Phiton™ technology utilizing nucleic acid dye structures engineered for minimal spillover and spread.

Excitation: 568 nm
Emission: 568 nm
Laser: 561 nm (Yellow)

Target Information

CD85j belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at chromosome region 19q13.4.
It has extracellular immunoglobulin domains, a transmembrane domain, and ITIM motifs that transmit inhibitory signals.
Expressed on immune cells, binding to MHC class I molecules to regulate immune activation and prevent autoreactivity.
Multiple transcript variants encoding different isoforms exist.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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