Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Red 725, eBioscience

Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.1 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT/LIR/MIR family and is a single transmembrane glycoprotein with a long cytoplasmic domain containing four ITIMs that signal through SHP-1 interactions.
Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells, including subsets of NK, T, and B cells. It has been correlated with leukemias (ALL, CLL) and viral infections such as HIV, EBV, and CMV. Ligands include MHC Class I molecules such as HLA-G, A, F, B27, E, and F.

The HP-F1 antibody reduces CD16-dependent cytolytic activity of NK cells. Each product contains one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral cells at ≤5 µL (0.1 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.

NovaFluor dyes are not compatible with DNA intercalating viability dyes (propidium iodide, 7-AAD, DAPI). Recommended viability dyes: Invitrogen LIVE/DEAD Fixable Dead Cell stains.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01). Add 5 µL per sample for optimal staining. For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, utilizing novel nucleic acid dye structures engineered for minimal spectral spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

This gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at 19q13.4. The encoded protein is part of the subfamily B class of LIR receptors, containing extracellular immunoglobulin domains, a transmembrane domain, and ITIM motifs. It is expressed on immune cells, binds MHC class I molecules, and transduces inhibitory signals to regulate immune responses and prevent autoreactivity. Multiple transcript variants encoding different isoforms exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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