Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Red 700, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.1 µg)/test

Product Specifications

Available Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600

Product Specific Information

The monoclonal antibody HP-F1 recognizes CD85j (ILT2, LILRB1, LIR1), a member of the ILT/LIR/MIR family.
CD85j is a single transmembrane glycoprotein with four ITIMs that interact with SHP-1 for inhibitory signaling.
It is expressed on myeloid cells (monocytes, dendritic cells) and some lymphoid cells (NK, T, B cell subsets).
Expression correlates with leukemias such as ALL and CLL and plays a role in viral infections (HIV, EBV, CMV).
Ligands include MHC Class I molecules (HLA-G, A, F, B27, E, F).
HP-F1 antibody reduces CD16-dependent cytolytic activity of NK cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested for human peripheral blood cells at 5 µL (0.25 µg) per test.
A test is defined as the amount of antibody that will stain a cell sample in 100 µL final volume.
Cell number range: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample to antibody cocktails before mixing with cells.
  • For single-color controls: add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescence with reduced spillover and spread.

Excitation: 639 nm
Emission: 700 nm
Laser: 633–640 nm (Red)

Target Information

The CD85j gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at chromosome 19q13.4.
It encodes a subfamily B class receptor with 2–4 extracellular Ig domains, a transmembrane region, and cytoplasmic ITIMs.
Expressed on immune cells, it binds MHC class I molecules and transduces inhibitory signals to regulate immune activation.
This receptor modulates inflammatory responses and cytotoxicity, helping prevent autoreactivity.
Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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