Thermo Fisher Scientific IL3RA/CD123 Recombinant Rabbit Monoclonal Antibody (IL3RA, 2947R)
Recombinant rabbit monoclonal antibody against human IL3RA/CD123. IHC(P) 및 Peptide Array에 사용 가능. HEK293 발현 시스템 기반으로 생산되며, Protein A/G 정제. PBS buffer, 무보존제, -20°C 이하 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.25–0.5 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | IL3RA, 2947R |
| Immunogen | Recombinant fragment of human IL3RA protein (around aa 26–171) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody tested in direct ELISA.
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and forms a heterodimer with a unique alpha chain paired with the common beta (βc or CDw131) subunit.
IL-3Ra mediates signal transduction of Interleukin-3. Heterodimerization of IL-3Ra and IL-3Rb chains is induced by Interleukin-3, which is necessary for receptor activation but not for high-affinity binding.
IL-3Ra subunits are reported to be overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells, making them potential targets for novel therapeutic agents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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