Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Brilliant Ultra Violet 496, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563 / 615 / 661 / 737
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue, Red, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 780
• Request custom conjugation available

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells.

Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions. It is widely used as a phenotypic marker for mouse T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤1.0 µg/test (100 µL final volume)
• Recommended Titration: Optimize empirically between 10⁵–10⁸ cells/test

Fluorochrome Information:
Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm and is designed for cytometers with a 355 nm UV laser. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple polymer dyes (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples may be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness.

Excitation: 350 nm
Emission: 496 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, which associate with cytoplasmic signaling molecules via tyrosine-based motifs. CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells.

Defects in the CD3 gene are linked to CD3 immunodeficiency. CD3 expression is crucial in T cell activation, proliferation, and survival.

For Research Use Only. Not for diagnostic procedures or resale without authorization.