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Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Brilliant Ultra Violet 395, eBioscience

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인간 IgM μ 사슬에 특이적인 단클론 항체로, B 세포 발달 단계 분석에 적합합니다. Brilliant Ultra Violet 395 형광체 결합으로 UV 레이저(355 nm) 검출 시스템에 최적화되었습니다. 흐름 세포 분석용으로 사전 희석되어 있으며, 비특이적 염색 최소화를 위해 citrate 항응고제 사용을 권장합니다.

카탈로그번호: 363-9998-4x (2개 옵션)
브랜드: Thermo Fisher Scientific
카테고리: Monoclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific 363-9998-42 IgM Monoclonal Antibody (SA-DA4), Brilliant Ultra Violet 395, eBioscience 100 tests pk판매 단위 pk ·

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Thermo Fisher Scientific 363-9998-41 IgM Monoclonal Antibody (SA-DA4), Brilliant Ultra Violet 395, eBioscience 25 tests pk판매 단위 pk ·

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Thermo Fisher Scientific · Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

Application: Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

항목	내용
Species Reactivity	Human
Host / Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Ultra Violet™ 395, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	SA-DA4
Conjugate	Brilliant Ultra Violet™ 395
Excitation / Emission Max	347 / 399 nm
Form	Liquid
Concentration	5 µL/test
Purification	Affinity chromatography
Storage Buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage Conditions	4°C, store in dark, DO NOT FREEZE
Shipping Conditions	Wet ice
RRID	AB_3093190

Additional Available Formats

Unconjugated
APC
Biotin
Brilliant UV 737
Brilliant Violet 480
Brilliant Violet 605
Brilliant Violet 786
eFluor 450
FITC
PE
PE-Cyanine7
PerCP-eFluor 710
Super Bright 436
Super Bright 600
Custom conjugation available

Product Specific Information

The SA-DA4 monoclonal antibody reacts with the μ heavy chain of human IgM and does not cross-react with other immunoglobulin classes (IgD, IgG, IgA).
IgM is expressed intracellularly during early B lymphopoiesis and on the surface of mature B cells.

Non-B cell staining variability may occur due to donor differences or anticoagulant type. Sodium citrate is recommended as the anticoagulant to minimize non-specific staining.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry of normal human peripheral blood cells
Recommended Use: 5 µL (0.25 µg) per test in 100 µL staining volume (10⁵–10⁸ cells/test)

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is optimized for cytometers equipped with a 355 nm UV laser. Ensure compatibility before use.

When using multiple polymer-based dyes (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to reduce non-specific polymer interactions.