Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet 496, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet 496, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1.0 µg)/test

Species Reactivity

• Chimpanzee
• Human

Product Specifications

Available Formats

• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant Violet 650
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Super Bright 780
• Request custom conjugation available

Product Specific Information

The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. Along with other CD3 subunits (gamma and delta), the epsilon chain is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells; use at 5 µL (1.0 µg) per test
• Recommended Cell Range: 10⁵–10⁸ cells/test

Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm and is designed for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific polymer interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness.

Excitation: 350 nm
Emission: 496 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 subunit complex is crucial for transducing antigen-recognition signals into the cytoplasm of T cells and regulating TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are encoded on human chromosome 11 and belong to the immunoglobulin superfamily.

• CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes.
• Not expressed on B or NK cells.
• Decreased CD3+ T lymphocytes may be observed in certain autoimmune diseases.
• Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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