Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), Brilliant Ultra Violet 496, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 737, 805
• Brilliant Violet 421, 480, 650
• eFluor 450, 506, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600, 702, 780
• Request custom conjugation

Product Specific Information

The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly and surface expression. CD3 is expressed by thymocytes and mature T cells.
Binding of 17A2 to CD3 triggers intracellular signaling leading to activation and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes (≤ 1.0 µg/test, 100 µL final volume)
  • Recommended to titrate antibody for optimal performance
  • Typical cell number: 10⁵–10⁸ cells/test

Fluorochrome Information:
Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm and is optimized for UV (355 nm) laser cytometers.
Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation: 350 nm
Emission: 496 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD3 subunit complex transduces antigen-recognition signals into T cells and regulates TCR surface expression.
CD3 subunits (gamma, delta, epsilon, zeta) are part of the immunoglobulin superfamily encoded on chromosome 11.
They associate with cytoplasmic signaling molecules via tyrosine-based motifs.

• CD3 is expressed on 68–82% of peripheral T lymphocytes and 65–85% of thymocytes.
• Not expressed on B or NK cells.
• CD3 gene defects are linked to CD3 immunodeficiency.
• May play roles in TCR-induced growth arrest, survival, and proliferation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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