Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. These antigens, associated with beta 2-microglobulin, are expressed by all human nucleated cells and play a central role in cell-mediated immune response and tumor surveillance.
W6/32 mAb recognizes a non-polymorphic epitope shared among HLA-A, B, and C loci, immunoprecipitating both 43 kDa and 11–12 kDa chains. Cross-reactivity is observed in baboon, rhesus, and cynomolgus monkey.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use at 5 µL (1.0 µg) per test in a final volume of 100 µL. Suitable for 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Ensure instrument compatibility with this fluorochrome.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and associated with beta2 microglobulin. They are critical for immune recognition by cytotoxic T cells, contributing to antiviral and antitumor defense.
HLA and MHC antibodies are essential tools in immunopeptidomics, aiding in neoantigen identification and characterization via high-performance liquid chromatography and tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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