Thermo Fisher Scientific RASGRP3 Monoclonal Antibody (OTI4G9), TrueMAB
RASGRP3 단백질을 인식하는 Mouse Monoclonal Antibody로, Western Blot과 IHC(P)에서 검증됨. Human 시료에 반응하며, Lyophilized 형태로 제공. PBS buffer(8% trehalose)로 안정화되어 있으며, 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 380–690 of human RASGRP3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Members of the RAS subfamily of GTPases function as molecular switches in signal transduction, cycling between inactive GDP- and active GTP-bound states.
Guanine nucleotide exchange factors (GEFs), such as RASGRP3, act as RAS activators by promoting GTP binding and maintaining the active state, serving as a key link between cell surface receptors and RAS activation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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