Thermo Fisher Scientific GFAP Monoclonal Antibody (N206B/9), FITC
GFAP 단백질 검출용 FITC 결합 단일클론 항체로 인간, 생쥐, 랫트 반응성. 면역형광, 면역조직화학, 웨스턴 블롯 등에 사용 가능. 단백질 G 정제, 액상 형태, 4°C 암소 보관. 뇌 신경교세포 표지 및 연구용 특화.
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Thermo Fisher Scientific GFAP Monoclonal Antibody (N206B/9), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N206B/9 |
| Immunogen | Synthetic peptide amino acids 411–422 (KTVEMRDGEVIK) of human GFAP |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932089 |
Additional Formats
- APC conjugate: MA5-45634
- PerCP conjugate: MA5-45636
- Custom conjugation available upon request
Product Specific Information
- 100% identical in rat and mouse.
- 1 µg/mL of MA5-45635 detects GFAP R416WT in 20 µg of rat brain lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP as secondary antibody.
- Detects approximately 50 kDa.
- Does not cross-react with GFAP-R416W or other proteins (based on KO validation).
- Formerly sold as clone S206B-9.
Target Information
GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein, specifically expressed in astrocytes and certain astroglia of the CNS, satellite cells, and non-myelinating Schwann cells. It serves as a marker for astrocytic cells and is expressed in various brain tumors derived from astrocytes. GFAP is also found in lens epithelium, Kupffer cells, and some salivary tumor cells. Mutations in GFAP cause Alexander disease, a rare astrocytic disorder. Alternative splicing produces multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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