Thermo Fisher Scientific GFAP Monoclonal Antibody (N206A/8), PerCP
GFAP 단백질 검출용 PerCP 결합 단클론 항체로 인간, 생쥐, 랫트 반응성을 가짐. Western blot, IHC, ICC/IF에 사용 가능. 단백질 G로 정제된 액상 형태이며 보존제가 없음. 4°C에서 보관, 신경교세포 및 뇌종양 연구에 적합.
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Thermo Fisher Scientific GFAP Monoclonal Antibody (N206A/8), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N206A/8 |
| Immunogen | Synthetic peptide amino acids 411–422 (KTVEMRDGEVIK) of human GFAP |
| Conjugate | PerCP |
| Excitation/Emission Max | 482/675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.46 mM phosphate / 2.48 mM MES, pH 7.4, with 2 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932087 |
Additional Formats
Product Specific Information
- 100% identical in rat and mouse.
- 1 µg/mL of MA5-45633 detects GFAP in 20 µg of rat brain lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP as secondary antibody.
- Detects approximately 50 kDa.
- Cross-reacts with GFAP-R416W and other GFAP mutant proteins.
- Formerly sold as clone S206A-8.
Target Information
GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein expressed in astrocytes and certain astroglia of the CNS, satellite cells of peripheral ganglia, and non-myelinating Schwann cells. Neural stem cells also strongly express GFAP.
Antibodies to GFAP are useful markers for astrocytic cells and brain tumors derived from astrocytes. GFAP is found in lens epithelium, Kupffer cells, salivary tumors, and erythrocytes. It is used to distinguish astrocytes from other glial cells during development.
Mutations in GFAP cause Alexander disease, a rare astrocytic disorder. Alternative splicing produces multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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