Takara iDimerize Inducible Heterodimer System

Takara iDimerize Inducible Heterodimer System

The iDimerize Inducible Heterodimer System enables the creation and control of specific interactions between two different proteins. Proteins of interest are fused to the DmrA and DmrC binding domains, respectively, and dimerization is induced by adding the cell-permeant ligand A/C Heterodimerizer (identical to the AP21967 ligand) to the culture medium or by administering it in vivo.
Both plasmid and lentiviral (Lenti-X) vector formats are available.

iDimerize Inducible Heterodimer System (with Tet-On 3G technology)

A common challenge in ligand-dependent dimerization experiments is non-ligand-induced dimerization, which can occur when target proteins are expressed at high levels. This issue is particularly significant for membrane-bound proteins, where local concentrations can rapidly increase due to limited membrane space.

By combining iDimerize and Tet-On 3G technologies, these unwanted events can be minimized:

1. Use doxycycline (Dox) to adjust protein expression to physiologically relevant levels.
2. Induce dimerization by adding the dimerizer ligand to the culture medium.

주요 특징

• 특정 단백질 간 상호작용의 정밀한 제어 가능
• DmrA/DmrC 융합 단백질 기반의 유도성 이합화 시스템
• 세포 투과성 리간드(AP21967 동일체)로 이합화 유도
• 플라스미드 및 Lenti-X 벡터 포맷 제공
• Tet-On 3G 기술과 결합하여 발현 수준 조절 가능

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