Takara 5X One-Step PrimeScript III RT-qPCR Kit

Takara 5X One-Step PrimeScript III RT-qPCR Kit

제품 개요

The 5X One Step PrimeScript III RT-qPCR Kit contains a 5X master mix for one-step real-time, probe-based RT-qPCR using the 5′ nuclease method. The master mix includes the heat- and inhibitor-resistant reverse transcriptase PrimeScript III RTase, combined with an efficient, specific hot-start PCR enzyme TaKaRa Taq HS, in an optimized reaction buffer.
A quick and simple protocol allows reverse transcription and qPCR reactions to be performed in the same tube, reducing the risk of contamination.

5X concentration improves the limit of detection (LoD) of the one-step RT-qPCR assay without a preamplification step, saving time and cost. PrimeScript III RTase displays increased heat tolerance (up to 55°C) while maintaining the specificity and robustness of PrimeScript RT, enabling cDNA synthesis from RNA with complex secondary structures.
It is highly resistant to inhibitory substances such as heparin (blood) and humic acid (soil), allowing stable one-step real-time RT-qPCR on a wide variety of samples.

While One Step PrimeScript III RT-qPCR Mix often works well with crude RNA extracts, highly pure RNA samples are recommended if the target sequence is not detectable or if more accurate, stable analysis results are needed.
This kit is suitable for various applications such as gene expression analysis and RNA virus detection and is well-suited for multiplex real-time PCR.

주요 특징

• Fast, easy one-step RT-qPCR in a single tube using probe-based detection without preamplification
• Efficient, specific cDNA synthesis and amplification from complex RNA templates
• Suitable for a wide variety of samples, including crude RNA extracts
• Compatible with automation systems such as the SmartChip Real-Time PCR System

응용 분야

• Automated gene expression analysis
• Detection of RNA viruses
• Multiplex real-time PCR

추가 정보

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
See the Kit Components List under the Documents tab for component details.

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