Thermo Fisher Scientific IL-10 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

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Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-10. Anti-Murine IL-10-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mIL-10 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-10 (500-P60) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant mIL-10.

Western Blot:
To detect mIL-10 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant mIL-10 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package:
500-P60BT-1MG will be provided as 2 × 500 µg.

Target Information

IL-10 encodes a cytokine primarily produced by monocytes, with some production by lymphocytes. It plays a key role in immunoregulation and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival, proliferation, and antibody production, blocks NF-kappa B activity, and regulates the JAK-STAT signaling pathway. Knockout studies in mice indicate it is essential for intestinal immune regulation. Mutations are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.